Gene expression profiling of brains from bovine spongiform encephalopathy (BSE)-infected cynomolgus macaques
Priondiseases are deadly neurodegenerative problems whose pathogenesis mechanisms aren’t totally understood. On this context, the evaluation of gene expression alterations occurring in prion-infected animals represents a robust instrument which will contribute to unravel the molecular foundation of priondiseases and subsequently uncover novel potential targets for analysis and therapeutics.
Right here we current the primary large-scale transcriptional profiling of brains from BSE-infected cynomolgus macaques, that are a superb mannequin for humanprion problems.RESULTSThe research was carried out utilizing the GeneChip Rhesus Macaque Genome Array and revealed 300 transcripts with expression adjustments larger than twofold.
Amongst these, the bioinformatics evaluation recognized 86 genes with identified features, most of that are concerned in mobile growth, cell demise and survival, lipid homeostasis, and acute section response signaling. RT-qPCR was carried out on chosen gene transcripts in an effort to validate the differential expression in contaminated animals versus controls.
The outcomes obtained with the microarray know-how had been confirmed and a gene signature was recognized. In short, HBB and HBA2 had been down-regulated in contaminated macaques, whereas TTR, APOC1 and SERPINA3 had been up-regulated.CONCLUSIONSSome genes concerned in oxygen or lipid transport and in innate immunity had been discovered to be dysregulated in prion contaminated macaques.
Human pig Diseases
These genes are identified to be concerned in different neurodegenerative problems resembling Alzheimer’s and Parkinson’s illnesses. Our outcomes might facilitate the identification of potential illness biomarkers for a lot of neurodegenerative illnesses.
MicroRNA profiling in canine multicentric lymphoma
Lymphoma is the commonest hematopoietic tumour in canines and is remarkably just like the humandisease. Tumour biomarker discovery is offering new instruments for diagnostics and predicting therapeutic response and scientific end result.
MicroRNAs are small non-coding RNAs that take part in post-transcriptional gene regulation and their aberrant expression can influence genes concerned in most cancers.
The intention of this research was to characterize microRNA expression in lymph nodes and plasma from canines with multicentric B or T cell lymphoma in comparison with wholesome management canines. We additional in contrast expression between lymph nodes and corresponding plasma samples and assessed adjustments in expression at relapse in comparison with time of analysis.
Lastly, we investigated microRNAs for affiliation with scientific end result in sufferers handled with CHOP chemotherapy. A custom-made PCRarray was utilized to profile 38 canine goal microRNAs.
Quantification was carried out utilizing actual time RT-qPCR and relative expression was decided by the delta-delta Ct methodology. In lymph nodes, there have been 16 microRNAs with considerably altered expression for B cell lymphoma and 9 for T cell lymphoma.
In plasma, there have been 15 microRNAs altered for B cell lymphoma and three for T cell lymphoma. The vast majority of microRNAs didn’t have correlated expression between lymph node and plasma and solely eight microRNAs had been considerably totally different between analysis and relapse.
Colon disease tissue array |
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| CO809b | TissueArray | each | EUR 306 |
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Description: Colon disease tissue array, including pathology grade, TNM and clinical stage, 80 cases/80 cores, replacing CO809a |
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Thyroid disease tissue array |
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| TH961 | TissueArray | each | EUR 306 |
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Description: Thyroid disease tissue array, including TNM/Stage (AJCC 7th edition), 48 cases/ 96 cores |
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Cardiovascular disease tissue array |
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| CV481 | TissueArray | each | EUR 252 |
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Description: Cardiovascular disease tissue array, including TNM and clinical stage, 24 cases/48 cores |
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Lung disease spectrum tissue array |
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| LC487 | TissueArray | each | EUR 168 |
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Description: Lung disease spectrum tissue array, including TNM and pathology grade, 24 cases/48 cores |
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Colon disease spectrum tissue array |
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| BC05002b | TissueArray | each | EUR 306 |
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Description: Colon disease spectrum tissue array, including TNM, clinical stage and pathology grade, 79 cases/80 cores, replaced by BC05002c |
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Renal disease spectrum tissue array |
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| KD2084 | TissueArray | each | EUR 474 |
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Description: Renal disease spectrum tissue array, with stage and TNM info, 104 cases/208 cores |
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Penis disease spectrum tissue array |
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| PE2081 | TissueArray | each | EUR 546 |
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Description: Penis disease spectrum tissue array, including TNM, clinical stage and pathology grade, 208 cases/ 208 cores |
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Liver disease spectrum tissue array |
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| LV20812b | TissueArray | each | EUR 474 |
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Description: Liver disease spectrum tissue array, including HBV infection information, pathology grade, TNM and clinical stage, 79 cases/192 cores, replacing LV20812a |
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Ovary disease spectrum tissue array |
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| OV1002a | TissueArray | each | EUR 306 |
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Description: Ovary disease spectrum tissue array, 99 cases/100 cores, replacing OV1002 |
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Cancer MicroRNA qPCR Array |
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| RA610A-1 | SBI | 10 profiles | EUR 1406.4 |
Breast disease spectrum tissue array |
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| BR963c | TissueArray | each | EUR 306 |
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Description: Breast disease spectrum tissue array, including TNM, clinical stage and pathology grade, 48 cases/96 cores, replacing BR963a |
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Tonsil disease spectrum tissue array |
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| OR1002a | TissueArray | each | EUR 306 |
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Description: Tonsil disease spectrum tissue array, containing pathology grade, TNM and clinical stage, 50 cases/100 cores, replacing OR1002 |
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Heart disease and heart tissue array |
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| BC30013a | TissueArray | each | EUR 270 |
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Description: Heart disease and heart tissue array, 18 cases/36 cores, replacing BC30013 |
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Thyroid disease spectrum tissue array |
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| TH201 | TissueArray | each | EUR 198 |
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Description: Thyroid disease spectrum tissue array, including TNM, clinical stage, 20 cases/ 20 cores |
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Thyroid disease spectrum tissue array |
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| TH807 | TissueArray | each | EUR 306 |
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Description: Thyroid disease spectrum tissue array, 75 cases/80 cores |
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Heart disease and normal tissue array |
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| BC30013 | TissueArray | each | EUR 270 |
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Description: Heart disease and normal tissue array, replacing BC30011, 18 cases/36 cores, replaced by BC30013a |
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Prostate disease spectrum tissue array |
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| PR821 | TissueArray | each | EUR 306 |
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Description: Prostate disease spectrum tissue array, including adenocarcinoma,metastatic carcinoma, hyperplasia,chronic prostatitis, NAT and normal tissue, including pathology grade, TNM /Stage ( AJCC 8th version), Gleason grade and Gleason score, 82 cases/82 cores (core size 1.5mm), replacing PR8011b |
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Breast spectrum of disease tissue array |
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| BR480b | TissueArray | each | EUR 306 |
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Description: Breast spectrum of disease tissue array, 48 cases/48 cores |
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LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
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| RA900A-1 | SBI | 20 profiles | EUR 1020 |
Mouse LncRNA Profiler qPCR Array Kit (cDNA synthesis kit and qPCR array) 20 profiles |
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| RA930A-1 | SBI | 20 profiles | EUR 1020 |
Uterine cervix disease spectrum tissue array |
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| CR602a | TissueArray | each | EUR 270 |
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Description: Uterine cervix disease spectrum tissue array, including TNM, clinical stage and pathology grade, 60 cases/ 60 cores, replacing CR602 |
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Fibrous tissue disease spectrum tissue array |
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| SO2084 | TissueArray | each | EUR 474 |
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Description: Fibrous tissue disease spectrum tissue array, 104 cases/208 cores (core size 1.0mm) |
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Ureter and urethra disease spectrum tissue array |
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| UR1001 | TissueArray | each | EUR 414 |
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Description: Ureter and urethra disease spectrum tissue array, with stage, grade and TNM data, 50 cases/100 cores |
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Esophageal disease (progression) spectrum tissue array |
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| ES2081 | TissueArray | each | EUR 546 |
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Description: Esophageal disease (progression) spectrum tissue array, 103 cases/208 cores |
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Goiter and thyroiditis (Hashimoto disease) tissue array |
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| TH805 | TissueArray | each | EUR 270 |
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Description: Goiter and thyroiditis (Hashimoto disease) tissue array, 80 cases/80 cores |
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Lung cnacer (spectrum of disease) with lung tissue array |
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| LC1201b | TissueArray | each | EUR 306 |
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Description: Lung cnacer (spectrum of disease) with lung tissue array, including pathology grade, TNM and clinical stage, 118 cases/120 cores, replacing LC1201a |
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Common diseases of the lung, 15 cases (2.5mm) |
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| LUD151 | Pantomics | 1 | EUR 228 |
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Description: Lung disease tissue array, 7 types, 15 cases of normal, inflammatory and tumor tissues of the lung. |
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Common diseases of the lung, 24 cases (2mm) |
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| LUD481 | Pantomics | 1 | EUR 300 |
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Description: Lung disease tissue array, non-overlapping with LUD151, 24 cases of normal, chronic bronchitis, emphysema, TB and tumor tissues of the lung in duplicates. |
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Common diseases of the skin, 24 cases (2.5mm) |
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| SKD241 | Pantomics | 1 | EUR 228 |
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Description: Skin disease tissue array, 24 cases of normal, reactive and tumor tissues of the skin |
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Common diseases of the skin, 24 cases (2mm) |
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| SKD481 | Pantomics | 1 | EUR 265.2 |
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Description: Skin disease tissue array, 24 cases of reactive and tumor conditions of the skin in duplicates. |
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Multiple types of colon disease with colon tissue array |
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| CO245a | TissueArray | each | EUR 66 |
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Description: Multiple types of colon disease with colon tissue array, including crohn‘s disease, chronic inflammation of the colonic mucosa, ulcerative colitis, adenocarcinoma, 12 cases/24 cores (core size 1.5mm), replacing CO245 |
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Common diseases of the liver, 24 cases (2mm) |
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| LVD481 | Pantomics | 1 | EUR 300 |
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Description: Liver common disease tissue array, 24 cases of normal, hepatitis, viral related cirrhosis and tumor tissues of the liver in duplicates. |
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Progression of colon disease (IBD) with colon tissue array |
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| CO246 | TissueArray | each | EUR 66 |
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Description: Progression of colon disease (IBD) with colon tissue array, 5 cases of crohn's disease, 2 ulcerative colitis, 5 normal colon tissue, 12 cases/24 cores |
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Common diseases of the breast, 102 cases (1.5mm) |
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| BRD1021 | Pantomics | 1 | EUR 300 |
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Description: Breast disease tissue array containing 102 cases of normal breasts, fibrocystic changes, proliferative breast diseases (atypical) and various types and stages of breast cancers with AR, ER, PR, HER2, P53, EGFR, Ki67 IHC data. |
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Common diseases of the breast, 18 cases (2.5mm) |
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| BRD181 | Pantomics | 1 | EUR 154.8 |
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Description: Breast disease tissue array containing 18 cases of normal, hyperplasia and neoplastic breast tissues with AR, ER, PR, HER2, P53, EGFR, Ki67 IHC data. |
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Common diseases of the breast, 24 cases (2mm) |
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| BRD481 | Pantomics | 1 | EUR 264 |
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Description: Breast disease tissue array containing 24 cases of normal, hyperplasia and neoplastic breast tissues with AR, ER, PR, HER2, P53, EGFR, Ki67 IHC data. |
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Common diseases of the breast, 48 cases (2mm) |
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| BRD961 | Pantomics | 1 | EUR 300 |
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Description: Breast disease tissue array containing 48 cases of normal, hyperplasia and neoplastic breast tissues in duplicates with AR, ER, PR, HER2, P53, EGFR, Ki67 IHC data. |
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Common diseases of soft tissue, 24 cases (2.5mm) |
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| SFD241 | Pantomics | 1 | EUR 228 |
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Description: Soft tissue disease tissue array, 24 cases of normal, reactive and tumor conditions of various soft tissues |
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Common diseases of soft tissue, 24 cases (2mm) |
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| SFD481 | Pantomics | 1 | EUR 265.2 |
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Description: Soft tissue disease tissue array, 24 cases of reactive and tumor conditions of various soft tissues in duplicates. |
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Common diseases of the thyroid, 18 cases (2.5mm) |
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| THD181 | Pantomics | 1 | EUR 193.2 |
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Description: Thyroid disease tissue array, 6 types, 18 cases of normal, inflammatory and neoplastic conditions of the thyroid |
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Common diseases of the thyroid, 48 cases (1.5mm) |
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| THD961 | Pantomics | 1 | EUR 300 |
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Description: Thyroid disease tissue array, 96 cores, 48 cases of normal, inflammatory and neoplastic conditions of the thyroid in duplicates |
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Common diseases of the stomach, 24 cases (2mm) |
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| STD481 | Pantomics | 1 | EUR 265.2 |
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Description: Stomach disease tissue array, 48 cores, 24 cases of normal, inflammatory and neoplastic conditions of the stomach in duplicates |
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Lung disease spectrum tissue with lung tissue array (2015 WHO classification) |
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| BC04002b | TissueArray | each | EUR 306 |
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Description: Lung disease spectrum tissue with lung tissue array (2015 WHO classification), including pathology grade, TNM and clinical stage (small cell carcinoma reference AJCC 8th edition), 99 cases/100 cores, replacing BC04002a |
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Common diseases of the prostate, 48 cases (1.5mm) |
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| PRD961 | Pantomics | 1 | EUR 354 |
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Description: Prostate disease tissue array, 48 cases of normal, hyperplastic and neoplastic prostate tissues in duplicates. |
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Colon disease spectrum with normal tissue array as control |
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| CO2081b | TissueArray | each | EUR 546 |
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Description: Colon disease spectrum with normal tissue array as control, including TNM, clinical stage and pathology grade with MMR (MLH1/PMS2/MSH2/MSH6) IHC results, containing 34 cases of adenocarcinoma, 2 carcinoid, 20 metastatic carcinoma, 5 each of adenoma and polyp, 8 hyperplasia, 10 each of inflammation, 104 cases/ 208 cores, replacing CO2081a |
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Heart disease spectrum (heart tumor progression) tissue array |
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| HE1001 | TissueArray | each | EUR 306 |
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Description: Heart disease spectrum (heart tumor progression) tissue array, 50 cases/100 cores |
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Penis disease spectrum (penis tumor progression) tissue array |
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| PE601 | TissueArray | each | EUR 306 |
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Description: Penis disease spectrum (penis tumor progression) tissue array, 59 cases/60 cores |
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LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent) 20 profiles |
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| RA910A-1 | SBI | 20 profiles | EUR 1329.6 |
Multiple kidney disease with kidney tissue array (2016/ISUP WHO classification |
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| KD804a | TissueArray | each | EUR 258 |
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Description: Multiple kidney disease with kidney tissue array (2016/ISUP WHO classification, Grade mean Isup Grade), including pathoogy grade, TNM and clinical stage (reference AJCC 8th version), 75 cases/80 cores, replacing KD804 |
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Colon disease spectrum (colon cancer progression) tissue array |
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| CO808 | TissueArray | each | EUR 306 |
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Description: Colon disease spectrum (colon cancer progression) tissue array, including pathology grade, TNM and clinical stage, 76 cases/80 cores |
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Tonsil disease spectrum (tonsil tumor progression) tissue array |
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| OR1001a | TissueArray | each | EUR 306 |
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Description: Tonsil disease spectrum (tonsil tumor progression) tissue array, 50 cases/100 cores, replacing OR1001 |
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Vulval disease spectrum (vulva cancer progression) tissue array |
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| UV1001 | TissueArray | each | EUR 306 |
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Description: Vulval disease spectrum (vulva cancer progression) tissue array, 46 cases/100 cores |
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QPCR Kit DNA Lyme disease - EACH |
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| MOL8196 | Scientific Laboratory Supplies | EACH | EUR 1176.51 |
Kidney disease spectrum (kidney cancer progression) tissue array |
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| BC07001 | TissueArray | each | EUR 258 |
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Description: Kidney disease spectrum (kidney cancer progression) tissue array, including TNM and pathology grade, 40 cases/80 cores (core size 1.5mm) |
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Breast disease spectrum (breast cancer progression) tissue array |
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| BR2082c | TissueArray | each | EUR 546 |
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Description: Breast disease spectrum (breast cancer progression) tissue array, including pathology grade, TNM and clinical stage, IHC marker (ER, PR, HER2 and Ki-67), 186 cases/192 cores, replacing BR2082b |
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Hepatic disease spectrum (liver cancer progression) tissue array |
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| LV2081 | TissueArray | each | EUR 678 |
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Description: Hepatic disease spectrum (liver cancer progression) tissue array, 103 cases/208 cores |
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Rectal disease spectrum (rectum cancer progression) tissue array |
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| RE804 | TissueArray | each | EUR 306 |
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Description: Rectal disease spectrum (rectum cancer progression) tissue array, 75 cases/80 cores |
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Skin disease spectrum (dermatic cancer progression) tissue array |
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| SK2081 | TissueArray | each | EUR 546 |
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Description: Skin disease spectrum (dermatic cancer progression) tissue array, 102 cases/208 cores |
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Skin disease spectrum (dermatic cancer progression) tissue array |
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| SK805 | TissueArray | each | EUR 306 |
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Description: Skin disease spectrum (dermatic cancer progression) tissue array, 79 cases/80 cores |
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Thymus disease spectrum (thymic cancer progression) tissue array |
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| THY761 | TissueArray | each | EUR 306 |
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Description: Thymus disease spectrum (thymic cancer progression) tissue array, 76 cases/76 cores |
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Lung disease spectrum (pulmonary cancer progression) tissue array |
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| LC2083 | TissueArray | each | EUR 546 |
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Description: Lung disease spectrum (pulmonary cancer progression) tissue array, 193 cases/208 cores |
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Lung disease spectrum (pulmonary cancer progression) tissue array |
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| LC809 | TissueArray | each | EUR 306 |
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Description: Lung disease spectrum (pulmonary cancer progression) tissue array, 78 cases/80 cores |
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For B cell lymphoma, eight microRNAs had differential expression within the non-remission group in comparison with canines that accomplished CHOP in full remission. 4 of those microRNAs had been additionally altered in sufferers that died prior to one-year.
Kaplan-Meier survival curves for prime versus low microRNA expression revealed that 10 microRNAs had been correlated with progression-free survival and three with general survival. This research highlights microRNAs of curiosity for canine multicentric lymphoma.
Future targets embrace growth of microRNA panels that could be helpful as biomarkers with the intent to supply improved end result prediction to veterinary most cancers sufferers.