Conformation-specific prion antibody: mAb 15B3
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About monoclonal antibody 15B3
The mouse monoclonal antibody 15B3 is a conformation-specific antibody that recognizes the disease-specific form of the prion protein (PrPSc) only (Korth et al., 1997, Nazor et al., 2005).
The IgM isotype antibody is capable of detecting PrPSc in brain homogenates without the need for Proteinase K digestion. This is of considerable importance for its application in blood, as it has been shown that prion infectivity in blood is sensitive to protease digestion and therefore might not be detected in methods using protease digestion.
The recent publication by the group of Byron Caughey (Orrú et al., 2011) describes a new and very sensitive method for the detection of disease-specific prion proteins in blood or plasma. The eQuIC assay uses amplification of prions together with a concentration step with the Prionics 15B3 antibody.
Applications
mAb 15B3 is used in immunoprecipitation experiments followed by detection using Western blotting, FACS analysis and the real-time quaking-induced conversion procedure (Orrú et al., 2011). Additionally, mAb 15B3 can be used as a capture antibody in sandwich ELISAs.
A detailed immunoprecipitation protocol is sent with the product and is available on request.
Scientific publications
Korth C, Stierli B, Streit P, Moser M, Schaller O, Fischer R, Schulz-Schaeffer W, Kretzschmar H, Raeber A, Braun U, Ehrensperger F, Hornemann S, Glockshuber R, Riek R, Billeter M, Wuthrich K, Oesch B.
Prion (PrPSc)-specific epitope defined by a monoclonal antibody. Nature. 1997 Nov 6;390(6655):74-7. PubMed Abstract
Nazor KE, Kuhn F, Seward T, Green M, Zwald D, Purro M, Schmid J, Biffiger K, Power AM, Oesch B, Raeber AJ, Telling GC. Immunodetection of disease-associated mutant PrP, which accelerates disease in GSS transgenic mice. EMBO J. 2005 Jul 6;24(13):2472-80. Epub 2005 Jun 16. Download pdf
Orrú CD, Wilham JM, Raymond LD, Kuhn F, Schroeder B, Raeber AJ, Caughey B Prion disease blood test using immunoprecipitation and improved quaking-induced conversion.. MBio. 2011 May 10;2(3). pii: e00078-11. doi: 10.1128/mBio.00078-11. Download pdf
mAb 15B3
Prod. No. 01-040 (100 μg)
Prod. No. 01-041 (1 mg)
IP Buffer (2x)
Prod. No. 01-046 (150 ml)
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