PrioCHECK[HOCH]®[/HOCH] PRV gE 2.0
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About the PrioCHECK[HOCH]®[/HOCH] PRV gE 2.0
The PrioCHECK® PRV gE 2.0 can detect PRV infected pigs in an already vaccinated population provided that the pigs have been vaccinated with viruses that do not express the PRV glycoprotein E (gE). The significance of this test is that it establishes the basis for combined Pseudorabies vaccination-eradication programs. As a result of its high sensitivity, the PrioCHECK® PRV gE 2.0, was used in the Dutch Pseudorabies eradication program with successful results.
Test procedure
The PrioCHECK® PRV gE 2.0 is a qualitative blocking ELISA for the detection of antibodies in the glycoprotein E (gE) of the Pseudorabies virus (PRV) in pig sera. The test is based on two monoclonal antibodies (mAbs) that recognize different antigenic determinants on gE. The first mAb is pre-coated to microtiter plates that are delivered as part of the kit. The test sample, the inactivated PRV antigen and a second anti-gE mAb, are then incubated together in the pre-coated microtiter plates. The second anti-gE mAb is then conjugated to an enzyme that generates a color signal. After a washing step, the enzyme substrate is added to the wells of the plates. The signal is then measured and when no color is formed, antibodies in the sample have competed for the inactivated PRV antigen. With this result, the sample is positive for PRV.
Performance
The PrioCHECK® PRV gE 2.0 has been evaluated for its specificity and sensitivity. In over 4000 field sera collected from PRV negative herds, all scored negative using the PrioCHECK® PRV gE 2.0 diagnostic test. The high specificity of this test was again demonstrated by testing sera collected from pigs that were experimentally infected with a panel of over 10 common porcine micro-organisms. The negative results with these sera showed no cross-reactivity with antibodies against other micro-organisms. The chance of finding false positive results was therefore unlikely.
The high sensitivity of PrioCHECK® PRV gE 2.0 is also evidenced by a finding that all 440 positive sera - obtained from experimentally infected pigs as well as from pigs infected in the field - were correctly identified using the test. The test was also found to approximate the sensitivity of the virus neutralizing test, the gold standard for PRV detection. (Source: van Oirschot JT et al. J Virol Methods 1988; 22:191-206)
Selected reading
van Oirschot JT et al. J Virol Methods 1988; 22:191-206
Development of an ELISA for detection of antibodies to glycoprotein I of Aujeszky's disease virus: a method for the serological differentiation between infected and vaccinated pigs.
