Prionics®-Check PrioSTRIP SR*
*pending approval - in EU & NAFTA countries for sale for research purposes only, not for regulatory or surveillance purposes
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About the Prionics®-Check PrioSTRIP SR
Prionics®-Check PrioSTRIP SR is as simple and easy to use as the Prionics®-Check PrioSTRIP. It comes also in our unique 8-strip format and can handle a veritable stampede of tests - in sequence or in parallel. PrioSTRIP® SR is sophisticated and incredibly simple to use. In comparison with the Prionics®-Check PrioSTRIP, we further reduced the testing time to as little as 70 minutes from brain to result. Because it is so simple and fast it is also very cost-effective. Staff, equipment and consumable costs are reduced to a minimum.
Test procedure
- Preparation of the brain sample and isolation of the TSE prion protein
The test sample is a small piece of obex, a defined region of the brain stem. The homogenized brain sample is incubated with an optimized reagent mixture consisting of digestion enzymes and a buffer solution. This solution degrades the normal prion protein. Only the TSE-specific prion protein remains in the test sample. - Immunological detection of TSE protein
After a preparation step, the sample is transferred to the test plate and mixed with the first antibody, which is conjugated to blue latex beads. The PrioSTRIP® SR combs are lowered into the sample mixture and left in the wells for 20 minutes. Antibody-prion protein complexes migrate up the strips and are bound at the test line. Uncomplexed antibodies bind at the control line. The result is interpreted immediately after the incubation, either visually or automatically - with the PrioSCAN®.
Testing times
The entire test procedure for one sample takes as little as 70 minutes from brain to result. 470 homogenized samples can be tested in parallel by one person in less than two hours.
Performance
The Prionics®-Check PrioSTRIP SR has been subjected to an extensive evaluation with excellent results. The ability of the Prionics®-Check PrioSTRIP SR to detect scrapie in sheep and goat was tested on 281 brain homogenates of TSE positive small ruminants and 1038 negative small ruminant brain homogenates. Furthermore, the suitability of the test for the detection of BSE and atypical scrapie was tested on brain homogenates of 11 sheep and one goat. The Prionics®-Check PrioSTRIP SR recognized all samples with 100 % specificity and 100 % sensitivity.
