PrioCHECK[HOCH]®[/HOCH] SVDV Ab
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About the Ceditest[HOCH]®[/HOCH] SVDV
The PrioCHECK® SVDV Ab is the first commercially available SVDV ELISA. This established test has been successfully applied in the Netherlands for almost 10 years in a National Screening program for SVDV. It is the test of choice for the control of SVDV outbreaks in many European countries because of its proven high specificity and sensitivity. The sera are tested at a single dilution (1:5), incubations are carried out at room temperature and test results are available within 3 hours. Together, these factors make the PrioCHECK® SVDV Ab simple, easy to automate and therefore very suitable for screening large numbers of serum samples.
Test procedure
The PrioCHECK® SVDV Ab is a competitive double antibody sandwich ELISA for the detection of specific antibodies against the Swine Vesicular Disease Virus (SVDV) found in the serum and plasma of pigs. Plates are coated with anti-SVDV specific monoclonal antibodies to which an inactivated SVDV antigen is bound. The sample and the detection antibody - a monoclonal antibody labeled with an enzyme that generates a color signal - are then added. The SVDV-specific antibodies present in the test sample and the detection antibody compete for binding to the SVDV-antigen. The signal is then measured and when no color is formed, antibodies in the sample have competed for the available viral protein. With this result, the sample is positive for SVDV.
Performance
The PrioCHECK® SVDV Ab has been validated using the field sera of SVDV infected and non-infected pig herds in the Netherlands as well as the sera from experimentally infected animals. The results were compared with those of the reference sera supplied by the Community Reference Laboratory for Swine Vesicular Disease at the Institute for Animal Health, Pirbright Laboratory, UK. Sensitivity and specificity were determined using the virus neutralization test (VNT) as the gold standard. After testing over 5,000 sera, the PrioCHECK® SVDV Ab scored >99% specificity and >97% sensitivity. All international referenced sera scored consistently correct. Sera collected sequentially from pigs experimentally infected with SVDV isolates representing all current SVDV groups, were scored positive slightly earlier by the PrioCHECK® SVDV Ab than by the VNT.
(Source: Chénard G. et al. J. Virol. Methods 1998; 75:105-112)
Selected reading
Chénard G. et al. J. Virol. Methods 1998; 75:105-112
Validation of a monoclonal antibody-based ELISA to detect antibodies directed against Swine Vesicular Disease Virus.
