PrioCHECK[HOCH]®[/HOCH] PRV gB
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About the PrioCHECK[HOCH]®[/HOCH] PRV gB
The PrioCHECK® PRV gB is an excellent tool for monitoring the PRV-free status of pig herds because it detects the presence of all PRV virus strains including live vaccines and mutant strains.
The PrioCHECK® PRV gB is also suited for the detection of antibodies after vaccination; to determine the effectiveness of vaccination; and, to identify illegally vaccinated animals. With this convenient test, no pre-dilution of serum samples is needed. Moreover, sample incubation requirements are flexible as samples can be incubated either at room temperature or at 37°C.
The PrioCHECK® PRV gB delivers clear results; that is, the sample is either positive or negative. There is no grey area when interpretating test results.
Test procedure
The PrioCHECK® PRV gB is a blocking ELISA that detects antibodies against the Pseudorabies virus (PRV) glycoprotein B in pig serum samples. The samples are incubated in a microtiter plate coated with an inactivated PRV antigen. After washing, the plates are incubated with a monoclonal antibody (mAb) directed against an epitope located on glycoprotein B (gB) of the PRV. This mAb is pre-labeled with an enzyme that generates a color signal. The signal is measured and when no color is formed the sample contains anti-PRV antibodies that competed for the PRV antigen. With this result, the sample is positive for PRV.
Performance
The PrioCHECK® PRV gB has been subjected to both internal and external evaluations. Over 500 PRV negative serum samples and 172 positive samples of vaccinated pigs have been tested. The PrioCHECK® PRV gB scored a specificity of 99.6% and a sensitivity of 100%. Moreover, the results showed a distinctive separation between positive and negative samples, reducing the risk of false positive results.
The Veterinary Laboratory Agency (England) has tested 200 routine serum samples with the PrioCHECK® PRV gB. All samples, correctly, tested negative. The sensitivity of the PrioCHECK® PRV gB has also met the criteria set out by the European Commission: The international Aujezky's Disease Virus 1 (ADV1) reference serum scored positive with the test in a 1:8 dilution.
(Cedi-Diagnostics, unpublished data)
