PrioCHECK[HOCH]®[/HOCH] FMDV NS
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About the PrioCHECK[HOCH]®[/HOCH] FMDV NS
The PrioCHECK® FMDV NS is an ELISA that detects antibodies against the highly conserved non-structural (NS) protein of the FMD virus. The test can be therefore used for all species.
Test procedure
The ELISA test plates of the kit contain the FMDV nonstructural protein, 3ABC, captured by the coated anti-3ABC monoclonal antibody (mAb). A second mAb labeled with an enzyme that generates a color signal - the detection antibody - is then added. The reaction between the FMDV-NS antigen and the detection antibody is blocked by antibodies directed against the non-structural proteins present in the sample. Consequently, the sample is positive if no color develops.
Performance
In May 2004, the EU-funded FMDV research group validated six assays for the detection of antibodies to the non-structural proteins of the FMD virus (FMDV).
In the validation study 3551 sera were tested. Most of the sera were cattle samples (2579) originating from eight countries; the rest were sheep (703) and pig (269) sera. The research group validated the PrioCHECK® FMDV NS as the best commercial kit because of its high specificity and sensitivity. Its performance was comparable to or even better than that of the OIE index ELISA test, the NCPanaftosa test.
(Source: Brocchi E et al. Vaccine 2006 Nov 17; 24:6966-79)
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PrioCHECK[HOCH]®[/HOCH] FMDV NS
Prod. No. 7610440
Prod. No. 7610450
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Selected reading
Brocchi E et al. Vaccine 2006 Nov 17; 24:6966-79
Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of foot-and-mouth disease virus
