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PrioCHECK[HOCH]®[/HOCH] CSFV Ab 2.0

  • Easy to use, a simple three-step protocol
  • Highly specific test, eliminates possibility of cross-reactivity with BVDV and BDV
  • Effective detection of sub-clinical CSFV infections
  • Highly reliable; 100% correlation with Golden Standard (NPLA)

 

 

About the PrioCHECK[HOCH]®[/HOCH] CSFV Ab 2.0

The PrioCHECK® CSFV Ab 2.0 is a blocking ELISA that specifically detects antibodies against the A domain of the E2 protein of CSFV. This domain is more specific for CSFV than the B, C and D domain of the E2 protein. An important advantage of the test is that it does not cross-react with related viruses such as Bovine Viral Diarrhoea virus (BVDV) and Border Disease virus (BDV).

The test detects antibodies against high-, moderate- and low virulent CSFV-strains early after infection. Detection of antibodies elicited by low virulent CSFV strains is of paramount importance to trace sub clinical CSFV infections.

Test procedure

The PrioCHECK® CSFV Ab 2.0 is a blocking ELISA. In this test, the plates are coated with the E2 CSFV envelope protein. The key reagent is a mAb directed against an epitope located on the A domain of the envelope protein E2 of CSFV, making the test more specific for CSFV. The mAb is labeled with an enzyme that generates a color signal. The signal is measured and when no color is formed, then the sample containing anti-CSFV antibodies that competed for the A domain of the E2 protein is positive for CSFV.