PrioCHECK[HOCH]®[/HOCH] Brucella Ab
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About the PrioCHECK[HOCH]®[/HOCH] Brucella Ab
The PrioCHECK® Brucella Ab is an indirect ELISA for the detection of antibodies against Brucella abortus and Brucella melitensis in the serum and milk of cattle, sheep and goats.
Test procedure
The PrioCHECK® Brucella Ab involves 4 steps:
In a first step serum samples and milk samples are dispensed in the coated wells of a microtiterplate. Antibodies, directed against Brucella abortus and Brucella melitensis, present in the test sample, will bind to the antigen during incubation. The bound antibodies are detected using an anti-Ig monoclonal antibody, conjugated to an enzyme that generates a color signal. Color development will occur when specific antibodies against Brucella abortus or Brucella melitensis are present in the test sample. Color development indicates the presence of Brucella antibodies in the sample.
Performance
The PrioCHECK® Brucella Ab test results meet the criteria of the Office International de Epizooties (OIE) International Standard serum panel. In addition, the test meets the requirements of the EU as described in the EU directive 64/432.
