PrioCHECK BVDV RT-PCR
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About the PrioCHECK® BVDV RT-PCR
The PrioCHECK® BVDV RT-PCR detects BVDV infections in animals of all ages, including PI animals. The test is a real time RT-PCR for the detection of viral RNA in ear notch tissue and blood. It is possible to pool up to 12 ear notch samples and up to 50 blood samples, making the test very economical. After RNA purification pre-treatment, the pool size for ear notch tissue can be even increased to 24 samples. The PrioCHECK® BVDV RT-PCR has a high diagnostic and analytical sensitivity (ca. 10 RNA copies in tissue lysates). With an amplification time of only 100 minutes the test is very fast.
Test Procedure
Ear notch samples are easily lysed using the Ear Notch Lysis Buffer. The RNA contained in the sample is reverse transcribed using a BVDV specific primer and the resulting cDNA is subsequently amplified using Taqman probes. Reverse transcription and amplification are performed in one step. An internal RNA amplification control is included to check the perfomance of the reverse transcriptase.
When BVDV RNA is present in the sample, the taqman probe is cleaved, releasing the Reporter from the Quencher molecule. The resulting light signal is measured.
The PrioCHECK® BVDV RT-PCR is validated with most commonly used real time PCR instruments. All reagents needed for the detection of BVDV RNA are included in the kit.
Performance
The PrioCHECK® BVDV RT-PCR was tested extensively both in a field trial and in BVDV ring trials with excellent results. In the field trial, 558 negative samples of different origins were recognized correctly, resulting in a specificity of 100 %. The sensitivity was determined at 99.7 %; 576 out of 578 positive samples were detected correctly. The test works with both ear tissue lysates and blood samples.
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PrioCHECK® BVDV RT-PCR
Prod. No. 7710600
Ear Notch Lysis Buffer
Prod. No. 7710700
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