PrioCHECK® M. avium Ab porcine
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About the PrioCHECK® M. avium Ab porcine
With the PrioCHECK® M. avium Ab porcine appropriate information about the MAA status in pig herds can be obtained and, based on the results, corrective measures to improve the MAA status in the pig herd can be implemented. In a risk-based surveillance scheme using the PrioCHECK® M. avium Ab porcine, only a fraction of the pigs needs to be tested. This saves time and labor as no incision of the lymph nodes of each individual animal at slaughter needs to be made. The test delivers more accurate results of infections at the herd level, allowing efficient implementation of appropriate control measures.
Serology testing is an accepted method to address MAA infections. The PrioCHECK® M. avium Ab porcine is more sensitive than the routine pathological examination performed at the slaughterhouse. The specificity is also higher, because the test does not deliver false positive results due to lesions caused by other infective agents such as Rhodococcus equi.
With the fast and robust PrioCHECK® M. avium Ab porcine, that follows a short four step protocol, 90 prepared samples can be analyzed within 150 minutes. The test thus allows efficient testing of large numbers of samples. The same sample taken for testing other pig diseases such as Salmonella, Toxoplasma or Trichinella infections can also be used in the PrioCHECK® M. avium Ab porcine. The benefit: Tests can be easily combined and no additional sample has to be taken, allowing efficient addressing of the herd health status.
Test procedure
The PrioCHECK® M. avium Ab porcine is a diagnostic kit based on ELISA technology. It follows a four step protocol consisting of sample preparation, sample incubation, conjugate incubation and detection. Serum or meat juice samples are incubated in an ELISA plate coated with the polar lipid fraction antigen of M. avium subsp. hominissuis. Anti-M. avium antibodies present in the sample bind to the antigen. Plates are then washed and an anti-swine antibody labeled with horse radish peroxidase (HRPO) is used to detect antibodies bound to the M. avium antigen. Color development using tetramethyl-benzidine (TMB) substrate measured optically at a wavelength of 450 nm shows the presence of antibodies directed against Mycobacterium avium.
Performance
The PrioCHECK® M. avium Ab porcine is a herd tool, providing sensitivity and specificity data at herd level rather than that at the level of the individual animal. This is crucial for the success of control programs. The PrioCHECK® M. avium Ab porcine has shown a very good herd sensitivity (~ 90 % and higher) within implemented surveillance systems.
