PrioCHECK® Trichinella Ab
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About the PrioCHECK® Trichinella Ab
The PrioCHECK® Trichinella Ab is a highly sensitive and specific ELISA for the detection of antibodies against Trichinella in serum and meat juice samples of pigs. No cross reactivity with commonly found pig parasites has been observed.
Test procedure
The PrioCHECK® Trichinella Ab is a diagnostic kit based on ELISA technology. It follows a four step protocol consisting of sample preparation, sample incubation, conjugate incubation and detection.
Serum or meat juice samples are incubated in an ELISA plate coated with the Trichinella E/S antigen. Anti-Trichinella antibodies present in the sample bind to the antigen. Plates are then washed and an anti-swine antibody labeled with horse radish peroxidase (HRP) is used to detect antibodies bound to the E/S antigen. Color development using mittels tetramethyl-benzidine (TMB) substrate measured optically at a wavelength of 450 nm shows the presence of antibodies directed against Trichinella spp.
Performance
The PrioCHECK® Trichinella Ab showed a sensitivity and specificity of 100% in Prionics in-house validation study, using Trichinella negative and Trichinella positive serum samples from pigs. The diagnostic status of all used samples was confirmed by an E/S ELISA of a national reference laboratory in the European Union. No cross-reactivity with other commonly found pig parasites such as Ascaris, Trichuris, Hyostrongylus and Strongyloides was observed.
