PrioCHECK[HOCH]®[/HOCH] Salmonella Ab porcine
|
About the PrioCHECK[HOCH]®[/HOCH] Salmonella Ab porcine
The PrioCHECK® Salmonella Ab porcine is a fast, effective and economical test for large-scale screening of pigs and for application in control programs of Salmonella infections. The PrioCHECK® Salmonella Ab porcine uses purified lipopolysaccharide (LPS) O-antigen of S. Typhimurium and S. Choleraesuis. This makes the PrioCHECK® Salmonella Ab porcine specific for the serogroup B, C1 and D; the most common serotypes in Europe, Asia and America.
Test procedure
The PrioCHECK® Salmonella Ab porcine is an indirect ELISA for the detection of antibodies against Salmonella LPS O-antigens 1, 4, 5, 6, 7 and 12 found in pig serum, plasma and meat juice samples. Test samples are incubated in plates coated with the purified LPS isolated from S. Typhimurium and S. Choleraesuis at room temperature. Plates are then washed and an anti-Swine antibody labeled with an enzyme that generates a color signal is added. The signal is measured and if color develops the sample is positive for anti-Salmonella LPS antibodies. Color intensity is directly related to the amount of anti-Salmonella antibody present in the test sample.
Performance
The PrioCHECK® Salmonella Ab porcine was originally developed by the Danish Veterinary Institute (Nielsen et al., 1995) and has been successfully applied in the control program for Salmonella in pigs in Denmark. The Danish test is often used as the gold standard for the development of other Salmonella ELISAs (van der Heijden, 2001). The PrioCHECK® Salmonella Ab porcine uses the same critical components (antibody and antigen) and has been used with excellent results in many European countries. The test results show a 97% agreement with the results of the Danish ELISA and with those of two other commercially available ELISAs.
Go to
PrioCHECK[HOCH]®[/HOCH] Salmonella Ab porcine
Prod. No. 7610460
Prod. No. 7610480
Related Products
Download: Product List
Selected reading
Nielsen B et al. Vet Microbiol. 1995; 47:205-218
The serological response to Salmonella serovars typhimurium and infantis in experimentally infected pigs. The time course followed with an indirect anti-LPS ELISA and bacteriological examinations.
van der Heijden HM. Berl Munch Tierarztl Wochenschr. 2001; 114: 389-392.
First international ring trial of ELISAs for Salmonella-antibody detection in swine.
